Adipose-derived stem cells combined with gelatin sponge for repair of rabbit radial defects

doi:10.3969/j.issn.2095-4344.2013.34.004

Abstract

Abstract:

BACKGROUND: Adipose-derived stem cells discovered recently are a new kind of adult stem cells, and have a strong multi-differentiation capacity. However, there are rare studies concerning in vivo osteogenic capacity of adipose-derived stem cells.
OBJECTIVE: To investigate the effect of adipose-derived stem cells combined with gelatin sponge on repairing bone defects.
METHODS: Adipose-derived stem cells from rabbit inguinal fat pads were isolated and cultured, and then induced using an osteogenic medium containing bone morphogenetic protein 2 followed by injection of gelatin sponge. Radial defect models of rabbits were prepared. Compound of adipose-derived stem cells and gelatin sponge was implanted into the lesion side, while gelatin sponge alone was implanted into the contralateral side. Rabbits were killed at weeks 6 and 12 after bone defect repair for X-ray examination, CT scan, and hematoxylin- eosin staining.
RESULTS AND CONCLUSION: Lane-Sandhu X-ray and Lane-Sandhu histological scores after compound implantation were significantly higher than those after repair with gelatin sponge alone. It indicates that adipose-derived stem cells combined with gelatin sponge can promote bone defect healing of rabbits, showing an obvious osteogenic capacity in vivo.

Key words: biomaterials, tissue-engineered bone materials, adipose-derived stem cells, radial defects, bone morphogenetic protein, cell culture, histology, radiology

CLC Number:

R318

Liu Gang, Wang Ming-xing, Yan Chang-ming, Wang Yun-qing, Wei Xue-jie, Hu Zhao-li . Adipose-derived stem cells combined with gelatin sponge for repair of rabbit radial defects[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(34): 6083-6088.

Figures/Tables 5

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